[InSCREENeX] CHO Freezing Medium A

어스바이오는 InSCREENeX 한국 공식 대리점으로서 모든 제품을 전문적으로 취급 및 공급하고 있습니다.
InSCREENeX의 freezing medium 제품을 소개드립니다.
 

CHO Freezing Medium A는 InSCREENeX의 안정적인 재조합 CHO 세포(A형)의 동결 보존에 최적화되어 있습니다. CHO Freezing Medium A는 사전 혼합되어 바로 사용할 수 있는 상태로 제공됩니다.
 

Product Details:

Catalog Number:

• INS-SU-1023

Features and Applications:

• Cryopreservation Medium
• Ready-to-use
• 30ml

Characteristics:

• Ready-to-use
• Optimized for high viability
• Contains serum

 

Contents:

• 30ml Freezing Medium ready-to-use

 

Cell Lines:

• CHO cells (Type-A)

Composition:

• 70% (v/v) CHO Growth Medium A (INS-ME-1039) without Geneticin G418 Sulfat
• 20% (v/v) FBS/FCS
• 10% (v/v) DMSO

Storage:

• Store the products as follows if not indicated otherwise on the product label. Freezing Medium -18 to -20°C, avoid repeated freeze-thaw cycles. Aliquot if necessary. Please see the product label for the expiry date of your product.

Instructions:

Cryopreservation

• Aspirate medium.
• Wash with PBS and aspirate PBS.
• Add Trypsin/EDTA (TE) solution to the cells and incubate at room temperature or 37°C for 5-10min, or until the cells detach.
• Examine the cells under a microscope. When the cells start to detach, gently tap the side of the vessel to loosen the remaining cells.
• Resuspend cells in 2% FBS in PBS and transfer to a 15ml conical bottom tube.
• Centrifuge cells at 200*g for 4min.
• Aspirate supernatant and gently resuspend cell pellet in Freezing medium (approx. 1Mio. cells/ml).
• Transfer cell suspension into cryovial(s) and place them into a freezing container (“Mr.Frosty” or similar).
• Place the freezing container at-80°C for 16-24h.
• Transfer cryovials to liquid nitrogen vapor for long-term-storage.

 

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